PA5-97883

antibody from Invitrogen Antibodies

Targeting: ITGB1BP1 ICAP-1A, ICAP-1alpha, ICAP-1B, ICAP1, ICAP1A, ICAP1B

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-97883

Provider

Invitrogen Antibodies

Product name

ITGB1BP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

3.35 mg/mL

Storage

-20°C or -80°C if preferred

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ITGB1BP1 using a ITGB1BP1 Polyclonal antibody (Product # PA5-97883). Positive WB detected in: SH-SY5Y whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, PC-3 whole cell lysate, MCF-7 whole cell lysate. All lanes: ITGB1BP1 antibody at 1:2000. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 22 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ITGB1BP1 using a ITGB1BP1 Polyclonal antibody (Product # PA5-97883). Positive WB detected in: SH-SY5Y whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, PC-3 whole cell lysate, MCF-7 whole cell lysate. All lanes: ITGB1BP1 antibody at 1:2000. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 22 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of ITGB1BP1 in paraffin embedded human heart tissue using a ITGB1BP1 polyclonal antibody (Product # PA5-97883) at a dilution of 1:300. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.