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LearnA301-034A
antibody from Invitrogen Antibodies
Targeting: KDM5C
DXS1272E, JARID1C, MRX13, SMCX, XE169
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Chromatin Immunoprecipitation [1]
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- Product number
- A301-034A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- JARID1C Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 0.20 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Jarid1C by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Jarid1C antibody A301-034A (lot A301-034A-2) used for IP at 6 µg per reaction. Jarid1C was also immunoprecipitated by rabbit anti-Jarid1C antibody A301-035A. For blotting immunoprecipitated Jarid1C, A301-034A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse Jarid1C by western blot. Samples: Whole cell lysate (50, 15 µg) from HeLa and (50 µg) from 293T, Jurkat, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Jarid1C antibody A301-034A (lot A301-034A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Localization of Jarid1C Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-Jarid1C antibody (Product # A301-034A) and analyzed by DNA sequencing. The figure illustrates the peak distribution of Jarid1C binding within a 500 Kb region of chromosome 1 as detected using anti-Jarid1C antibody (Product # A301-034A). ChIP-seq validation performed by Diogenode, Denville, NJ.
