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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- A301-406A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anillin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Anillin by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Anillin antibody (Product # A301-406A; lot A301-406A-1) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Anillin by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Anillin antibody A301-406A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Anillin was also immunoprecipitated by rabbit anti-Anillin antibody A301-405A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Anillin by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Anillin (Cat. No. A301-406A) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Anillin by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Anillin antibody (Product # A301-406A) used for IP at 3 µg/mg lysate. Anillin was also immunoprecipitated by rabbit anti-Anillin antibody (Product # A301-405A). For blotting immunoprecipitated Anillin (Product # A301-406A) was used at 1.0 µg/mL. Detection: Chemiluminescence with exposure time of 3 seconds.
