HPA016700

antibody from Atlas Antibodies

Targeting: TREML1 dJ238O23.3, TLT1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA016700

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA016700, RRID:AB_1858262

Product name

Anti-TREML1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

KRKQGNRLGVCGRFLSSRVSGMNPSSVVHHVSDSG
PAAELPLDVPHIRLDSPPSFDNTTYTSLPLDSPSG
KPSLPAPSSLPPLPPKVLVCSKPVTYATVIFPGGN
KGGGTSCGPAQNPPNNQTPSS


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and tREML1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406014).

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human bone marrow and colon tissues using Anti-TREML1 antibody. Corresponding TREML1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Immunohistochemical staining of human bone marrow, colon, kidney and testis using Anti-TREML1 antibody HPA016700 (A) shows similar protein distribution across tissues to independent antibody HPA017860 (B).

Antibody #2 product nr

HPA017860

Antibody provider

Atlas Antibodies

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human bone marrow shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon shows low expression as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis using Anti-TREML1 antibody HPA016700.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney using Anti-TREML1 antibody HPA016700.

Sample type

HUMAN