11501-2-AP

antibody from Invitrogen Antibodies

Targeting: ASGR2 CLEC4H2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11501-2-AP

Provider

Invitrogen Antibodies

Product name

ASGR2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAKDFQDIQ QLSSEENDHP FHQGPPPAQP LAQRLCSMVC FSLLALSFNI LLLVVICVTG SQSAQLQAEL RSLKEAFSNF SSSTLTEVQA ISTHGGSVGD KITSLGAKLE KQQQDLKADH DALLFHLKHF PVDLRFVACQ MELLHSNGSQ RTCCPVNWVE HQGSCYWFSH SGKAWAEAEK YCQLENAHLV VINSWEEQKF IVQHTNPFNT WIGLTDSDGS WKWVDGTDYR HNYKNWAVTQ PDNWHGHELG GSEDCVEVQP DGRWNDDFCL QVYRWVCEKR RNATGEVA (1-287 aa encoded by BC017251)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.23 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human liver tissue were subjected to SDS PAGE followed by western blot with 11501-2-AP (ASGR2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 11501-2-AP ( ASGR2 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 11501-2-AP ( ASGR2 Antibody) at dilution of 1:200 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HepG2 cells were stained with 0.2ug ASGR2 antibody (11501-2-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ASGR2 (IP:11501-2-AP, 4ug; Detection:11501-2-AP 1:300) with L02 cells lysate 1800ug.