PAB28288

antibody from Abnova Corporation

Targeting: NME5 nm23-H5, RSPH23

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28288

Provider

Abnova Corporation

Product name

NME5 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant NME5.

Antigen sequence

FMFPEVIVEPIPIGQAAKDYLNLHIMPTLLEGLTE
LCKQKPADPLIWLADWLLKNNPNKPKLCHHPI


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate) Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with NME5 polyclonal antibody (Cat # PAB28288).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with NME5 polyclonal antibody ( Cat # PAB28288 ) at 1-4 ug/mL dilution shows positivity in plasma membrane & cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human fallopian tube with NME5 polyclonal antibody ( Cat # PAB28288 ) shows strong positivity in cilia of glandular cells at 1:1000 - 1:2500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)