GTX115780

antibody from GeneTex

Targeting: SH2B1 FLJ30542, SH2B

Antibody data

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Validation data

SH2B1 protein structure - GTX115780 shown in red.

Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) C2C12 whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with SH2B antibody (GTX115780) diluted at 1:2000.
KD Reagent Type: siRNA
Downregulation: >50%
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Prod no: Proprietary.
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
Antibody Lot Number: 41983

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) C2C12 whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with SH2B antibody (GTX115780) diluted at 1:2000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various tissue extracts (50 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SH2B antibody (GTX115780) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with SH2B antibody (GTX115780) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.