12226-1-AP

antibody from Invitrogen Antibodies

Targeting: SH2B1 FLJ30542, SH2B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12226-1-AP

Provider

Invitrogen Antibodies

Product name

SH2B1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MVQREELLS FMGAEEAAPD PAGVGRGGGV AGPPSGGGGQ PQWQKCRLLL RSEGEGGGGS RLEFFVPPKA SRPRLSIPCS SITDVRTTTA LEMPDRENTF VVKVEGPSEY IMETVDAQHV KAWVSDIQEC LSPGPCPATS PRPMTLPLAP GTSFLTRENT DSLELSCLNH SESLPSQDLL LGPSESNDRL SQGAYGGLSD RPSASISPSS ASIAASHFDS MELLPPELPP RIPIEEGPPA GTVHPLSAPY PPLDTPETAT GSFLFQGEPE GGEGDQPLSG YPWFHGMLSR LKAAQLALTG GTGSHGVFLV RQSETRRGEY VLTFNFQGKA KHLRLSLNEE GQCRVQHLWF Q (1-350 aa encoded by BC010704)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.29 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 12226-1-AP ( SH2B1 Antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 12226-1-AP (SH2B1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 12226-1-AP (SH2B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 12226-1-AP (SH2B1 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 12226-1-AP (SH2B1 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary tissue slide using 12226-1-AP ( SH2B1 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary tissue slide using 12226-1-AP ( SH2B1 Antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-SH2B1 (IP:12226-1-AP, 4ug; Detection:12226-1-AP 1:600) with HEK-293 cells lysate 3000ug.