Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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Validation data
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- Product number
- H00065220-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00065220-M01, RRID:AB_540693
- Product name
- Mouse Monoclonal NADK Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. NADK - NAD kinase
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references NAD kinase levels control the NADPH concentration in human cells.
Pollak N, Niere M, Ziegler M
The Journal of biological chemistry 2007 Nov 16;282(46):33562-71
The Journal of biological chemistry 2007 Nov 16;282(46):33562-71
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NADK Antibody (5F4) [H00065220-M01] - Analysis of NADK expression in transfected 293T cell line by NADK monoclonal antibody (M01), clone 5F4.Lane 1: NADK transfected lysate(49.2 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NADK Antibody (5F4) [H00065220-M01] - NADK monoclonal antibody (M01), clone 5F4 Analysis of NADK expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: NADK Antibody (5F4) [H00065220-M01] - Detection limit for recombinant GST tagged NADK is approximately 0.3ng/ml as a capture antibody.