AP8089a

antibody from Abgent

Targeting: PACSIN3 SDPIII

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP8089a

Provider

Abgent

Proper citation

Abgent Cat#AP8089a, RRID:AB_353995

Product name

PACSIN3 Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

The anti-PACSIN3 Pab (Cat. #AP8089a) is used in Western blot to detect PACSIN3 in mouse brain tissue lysate.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

PACSIN3 Antibody (M1) (Cat. #AP8089a) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PACSIN3 antibody detected the PACSIN3 protein (arrow).

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of PACSIN3 (arrow) using rabbit polyclonal PACSIN3 Antibody (M1) (Cat. #AP8089a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PACSIN3 gene.

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."

Primary Ab dilution

1:50~100