NBP1-88861
antibody from Novus Biologicals
Targeting: PARD3
ASIP, Baz, Bazooka, PAR3, PARD3A, PPP1R118
Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP1-88861 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-88861, RRID:AB_11056253
- Product name
- Rabbit Polyclonal PARD3/Par3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human PARD3/Par3 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Distinct Roles for Rac1 in Sertoli Cell Function during Testicular Development and Spermatogenesis.
MCAM contributes to the establishment of cell autonomous polarity in myogenic and chondrogenic differentiation.
Expression and prognostic value of the cell polarity PAR complex members in thyroid cancer.
Crumbs3 is essential for proper epithelial development and viability.
Heinrich A, Potter SJ, Guo L, Ratner N, DeFalco T
Cell reports 2020 Apr 14;31(2):107513
Cell reports 2020 Apr 14;31(2):107513
MCAM contributes to the establishment of cell autonomous polarity in myogenic and chondrogenic differentiation.
Moreno-Fortuny A, Bragg L, Cossu G, Roostalu U
Biology open 2017 Nov 15;6(11):1592-1601
Biology open 2017 Nov 15;6(11):1592-1601
Expression and prognostic value of the cell polarity PAR complex members in thyroid cancer.
Tuccilli C, Baldini E, Arlot-Bonnemains Y, Chesnel F, Sorrenti S, De Vito C, D'Armiento E, Antonelli A, Fallahi P, Watutantrige S, Tartaglia F, Barollo S, Mian C, Arcieri S, Mascagni D, Pironi D, Bononi M, Vergine M, Monti M, Filippini A, Ulisse S
International journal of oncology 2017 Mar 8;
International journal of oncology 2017 Mar 8;
Crumbs3 is essential for proper epithelial development and viability.
Whiteman EL, Fan S, Harder JL, Walton KD, Liu CJ, Soofi A, Fogg VC, Hershenson MB, Dressler GR, Deutsch GH, Gumucio DL, Margolis B
Molecular and cellular biology 2014 Jan;34(1):43-56
Molecular and cellular biology 2014 Jan;34(1):43-56
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: PARD3/Par3 Antibody [NBP1-88861] - Simple Western lane view shows a specific band for PARD3/Par3 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: PARD3/Par3 Antibody [NBP1-88861] - Electropherogram image(s) of corresponding Simple Western lane view. PARD3/Par3 antibody was used at 1:100 dilution on RT-4 lysate(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PARD3/Par3 Antibody [NBP1-88861] - Analysis in human cell lines A-549 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human colon shows distinct luminal membranous positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human cerebellum shows moderate positivity in neuropil.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human fallopian tube shows weak to moderate positivity in luminal membrane in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human skin shows weak to moderate cytoplasmic positivity in epidermal cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human kidney shows weak to moderate membranous positivity in cells in tubules.