M00253

antibody from Boster Biological Technology

Targeting: HMOX1 bK286B10, HO-1

Western blot (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M00253

Provider

Boster Biological Technology

Product name

Anti-Heme Oxygenase 1 HMOX1 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for HO 1/HMOX1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IHC, WB. Reactive species: Human, Mouse HO 1/HMOX1 information: Molecular Weight: 32819 MW; Subcellular Localization: Microsome . Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side ; Tissue Specificity: Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level).

Reactivity

Human, Mouse

Host

Rabbit

Antibody clone number

AEG-8

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human liver, using Heme Oxygenase 1 Antibody(M00253)HMOX1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HMOX1 Antibody (M00253)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image