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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [2]
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- Product number
- NBP1-31341 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-31341, RRID:AB_2118677
- Product name
- Rabbit Polyclonal HO-1/HMOX1/HSP32 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Modulation of Keap1/Nrf2/ARE Signaling Pathway by Curcuma- and Garlic-Derived Hybrids.
Merging memantine and ferulic acid to probe connections between NMDA receptors, oxidative stress and amyloid-β peptide in Alzheimer's disease.
Selenium Deficiency Is Associated with Pro-longevity Mechanisms.
A novel synthetic small molecule DMFO targets Nrf2 in modulating proinflammatory/antioxidant mediators to ameliorate inflammation.
The active metabolite of leflunomide, A77 1726, attenuates inflammatory arthritis in mice with spontaneous arthritis via induction of heme oxygenase-1.
RBM45 Modulates the Antioxidant Response in Amyotrophic Lateral Sclerosis through Interactions with KEAP1.
Serafini MM, Catanzaro M, Fagiani F, Simoni E, Caporaso R, Dacrema M, Romanoni I, Govoni S, Racchi M, Daglia M, Rosini M, Lanni C
Frontiers in pharmacology 2019;10:1597
Frontiers in pharmacology 2019;10:1597
Merging memantine and ferulic acid to probe connections between NMDA receptors, oxidative stress and amyloid-β peptide in Alzheimer's disease.
Rosini M, Simoni E, Caporaso R, Basagni F, Catanzaro M, Abu IF, Fagiani F, Fusco F, Masuzzo S, Albani D, Lanni C, Mellor IR, Minarini A
European journal of medicinal chemistry 2019 Oct 15;180:111-120
European journal of medicinal chemistry 2019 Oct 15;180:111-120
Selenium Deficiency Is Associated with Pro-longevity Mechanisms.
Yim SH, Clish CB, Gladyshev VN
Cell reports 2019 May 28;27(9):2785-2797.e3
Cell reports 2019 May 28;27(9):2785-2797.e3
A novel synthetic small molecule DMFO targets Nrf2 in modulating proinflammatory/antioxidant mediators to ameliorate inflammation.
Mathew G, Sharma A, Pickering RJ, Rosado CJ, Lemarie J, Mudgal J, Thambi M, Sebastian S, Jandeleit-Dahm KA, de Haan JB, Unnikrishnan MK
Free radical research 2018 Oct;52(10):1140-1157
Free radical research 2018 Oct;52(10):1140-1157
The active metabolite of leflunomide, A77 1726, attenuates inflammatory arthritis in mice with spontaneous arthritis via induction of heme oxygenase-1.
Moon SJ, Kim EK, Jhun JY, Lee HJ, Lee WS, Park SH, Cho ML, Min JK
Journal of translational medicine 2017 Feb 13;15(1):31
Journal of translational medicine 2017 Feb 13;15(1):31
RBM45 Modulates the Antioxidant Response in Amyotrophic Lateral Sclerosis through Interactions with KEAP1.
Bakkar N, Kousari A, Kovalik T, Li Y, Bowser R
Molecular and cellular biology 2015 Jul;35(14):2385-99
Molecular and cellular biology 2015 Jul;35(14):2385-99
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - analysis of HO-1 in HEK293 cell lysate using anti-HO-1 antibody. WB Image submitted by a verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody diluted at 1:1500.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - ELISA detection of recombinant full-length Heme Oxygenase 1 protein using as capture antibody at concentration of 5 ug/mL and as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Paraffin-embedded rat spleen antibody diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Paraffin-embedded human renal carcinoma diluted at 1:500.
