LS-C351853

antibody from LSBio

Targeting: ATP2A1 ATP2A, SERCA1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C351853

Provider

LSBio

Product name

ATP2A1 / SERCA1 Antibody (Internal) LS-C351853

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human, Mouse, Rat, Bovine, Rabbit

Host

Rabbit

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of SERCA1 expression in A549 (A); mouse muscle (B); rat muscle (C) whole cell lysates.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemical analysis of SERCA1 staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemical analysis of SERCA1 staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.