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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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- Product number
- GTX114442 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114442, RRID:AB_10619473
- Product name
- GNB1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Identification of up- and down-regulated proteins in doxorubicin-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of doxorubicin-associated resistance.
May EW, Lin ST, Lin CC, Chang JF, Hung E, Lo YW, Lin LH, Hu RY, Feng CL, Lin DY, Wu SB, Lee WC, Lyu KW, Chou HC, Chan HL
Pharmacological research 2014 Dec;90:1-17
Pharmacological research 2014 Dec;90:1-17
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ?g of whole cell lysate) A: mouse brain 10% SDS PAGE GTX114442 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: JurKat 10% SDS PAGE GTX114442 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GNB1 antibody detects GNB1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GNB1 protein stained by GNB1 antibody (GTX114442) diluted at 1:500.Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] (GTX631209) diluted at 1:20000.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of GNB1 protein from 293T whole cell extracts using 5 £gg of GNB1 antibody (GTX114442).Western blot analysis was performed using GNB1 antibody (GTX114442).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung papillory adenocarcinoma, using GNB1(GTX114442) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GNB1 antibody detects GNB1 protein at cell membrane in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. GNB1 antibody (GTX114442) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GNB1 antibody detects GNB1 protein at cell membrane in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. GNB1 antibody (GTX114442) diluted at 1:1000.
