MAB8120

antibody from R&D Systems

Targeting: SAMHD1 AGS5, HDDC1, Mg11, MOP-5, SBBI88

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB8120

Provider

R&D Systems

Product name

Human SAMHD1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human SAMHD1 in ELISAs.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

Q9Y3Z3


Isotype

IgG

Antibody clone number

883335

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human SAMHD1 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SAMHD1 Monoclonal Antibody (Catalog # MAB8120) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SAMHD1 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human SAMHD1 by Simple Western^TM. Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line, loaded at 0.5 mg/mL. A specific band was detected for SAMHD1 at approximately 70 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human SAMHD1 Monoclonal Antibody (Catalog # MAB8120) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.