NBP2-03285

antibody from Novus Biologicals

Targeting: SAMHD1 AGS5, HDDC1, Mg11, MOP-5, SBBI88

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-03285

Provider

Novus Biologicals

Product name

Mouse Monoclonal SAMHD1 Antibody

Antibody type

Monoclonal

Description

Affinity purified.

Reactivity

Human, Mouse, Rat, Canine, Simian

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

1 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

A viral kinase counteracts in vivo restriction of murine cytomegalovirus by SAMHD1.

Deutschmann J, Schneider A, Gruska I, Vetter B, Thomas D, Kießling M, Wittmann S, Herrmann A, Schindler M, Milbradt J, Ferreirós N, Winkler TH, Wiebusch L, Gramberg T
Nature microbiology 2019 Dec;4(12):2273-2284

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Human cytomegalovirus overcomes SAMHD1 restriction in macrophages via pUL97.

Businger R, Deutschmann J, Gruska I, Milbradt J, Wiebusch L, Gramberg T, Schindler M
Nature microbiology 2019 Dec;4(12):2260-2272

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SAMHD1 Posttranscriptionally Controls the Expression of Foxp3 and Helios in Human T Regulatory Cells.

Kim YC, Kim KK, Yoon J, Scott DW, Shevach EM
Journal of immunology (Baltimore, Md. : 1950) 2018 Sep 15;201(6):1671-1680

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SAMHD1 Antibody (3F5) [NBP2-03285] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMHD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMHD1.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SAMHD1 Antibody (3F5) [NBP2-03285] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SAMHD1 monoclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SAMHD1 Antibody (3F5) [NBP2-03285] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SAMHD1 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SAMHD1 Antibody (3F5) [NBP2-03285] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SAMHD1 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SAMHD1 Antibody (3F5) [NBP2-03285] - Staining of paraffin-embedded Human pancreas tissue using anti-SAMHD1 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SAMHD1 Antibody (3F5) [NBP2-03285] - Staining of paraffin-embedded Human lymphoma tissue using anti-SAMHD1 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SAMHD1 Antibody (3F5) [NBP2-03285] - Staining of paraffin-embedded Human tonsil using anti-SAMHD1 mouse monoclonal antibody.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: SAMHD1 Antibody (3F5) [NBP2-03285] - Analysis of Hela cells, using anti-SAMHD1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: SAMHD1 Antibody (3F5) [NBP2-03285] - Analysis of Jurkat cells, using anti-SAMHD1 antibody,(Red), compared to a nonspecific negative control antibody,(Blue).