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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00000053-M01 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ACP2 Monoclonal Antibody (M1-4A12)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Sequence of this protein is as follows: MAGKRSGWSR AALLQLLLGV NLVVMPPTQA RSLRFVTLLY RHGDRSPVKT YPKDPYQEEE WPQGFGQLTK EGMLQHWELG QALRQRYHGF LNTSYHRQEV YVRSTDFDRT LMSAEANLAG LFPPNGMQRF NPNISWQPIP VHTVPITEDR LLKFPLGPCP RYEQLQNETR QTPEYQNESS RNAQFLDMVA NETGLTDLTL ETVWNVYDTL FCEQTHGLRL PPWASPQTMQ RLSRLKDFSF RFLFGIYQQA EKARLQGGVL LAQIRKNLTL MATTSQLPKL LVYSAHDTTL VALQMALDVY NGEQAPYASC HIFELYQEDS GNFSVEMYFR NESDKAPWPL SLPGCPHRCP LQDFLRLTEP VVPKDWQQEC QLASGPADTE VIVALAVCGS ILFLLIVLLL TVLFRMQAQP PGYRHVADGE DHA
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- M1-4A12
- Vial size
- 100 µg
- Concentration
- See Label
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ACP2 over-expressed 293 cell line, cotransfected with ACP2 Validated Chimera RNAi (Cat # H00000053-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ACP2 monoclonal antibody (M01), clone M1-4A12 (Cat # H00000053-M01). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ACP2 over-expressed 293 cell line, cotransfected with ACP2 Validated Chimera RNAi (Cat # H00000053-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ACP2 monoclonal antibody (M01), clone M1-4A12 (Cat # H00000053-M01). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ACP2 expression in transfected 293T cell line by ACP2 monoclonal antibody (M01), clone M1-4A12.Lane 1: ACP2 transfected lysate(48.3 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ACP2 is 1 ng/mL as a capture antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to ACP2 on HeLa cell. Antibody concentration is 10 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to ACP2 on HeLa cell. Antibody concentration is 10 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to ACP2 on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration is 5 µg/mL.
