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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [2]
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- Product number
- PA5-92365 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RRM2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.51 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Yap is essential for uterine decidualization through Rrm2/GSH/ROS pathway in response to Bmp2.
Yu HF, Yang ZQ, Xu MY, Huang JC, Yue ZP, Guo B
International journal of biological sciences 2022;18(6):2261-2276
International journal of biological sciences 2022;18(6):2261-2276
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RRM2 in extracts of various cell lines using RRM2 Polyclonal Antibody (Product # PA5-92365) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of RRM2 was performed in A549 cells using RRM2 Polyclonal Antibody (Product # PA5-92365). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of RRM2 was performed in 200 µg extracts of HeLa cells using RRM2 Polyclonal Antibody (Product # PA5-92365). Western blot was performed from the immunoprecipitate using RRM2 Polyclonal Antibody at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rrm2 was direct target of Yap function in stromal differentiation and its expression was regulated by Bmp2 dependent on Yap. (A) Immunofluorescence analysis of Rrm2 expression in decidual cells (N = 3 per group). Scale bar, 60 um. (B and C) Rrm2 mRNA and protein expression after treatment with Yap siRNA or Verteporfin (N = 3 per group). (D) Schematic diagram exhibited Yap/Tead binding sites and mutation in Rrm2 promoter region. (E) Relative luciferase activity after pGL6-Rrm2 or pGL6-Rrm2-mutant plasmid was co-transfected with Yap siRNA (N = 6 per group). (F) Relative luciferase activity after introduction of pGL6-Rrm2 or pGL6-Rrm2-mutant plasmid along with an addition of Yap inhibitor Verteporfin (N = 6 per group). (G-J) Overexpression of Rrm2 rescued the defect of Prl8a2 and Prl3c1 expression as well as ALP activity elicited by Yap inactivation (N = 5 per group). (K-N) Blockage of Yap abrogated the regulation of Bmp2 on Rrm2 mRNA and protein expression (N = 3 per group).
