TA502891

antibody from OriGene

Targeting: ETF1 ERF, ERF1, RF1, SUP45L1, TB3-1

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA502891

Provider

OriGene

Proper citation

OriGene Cat#TA502891, RRID:AB_11125468

Product name

ETF1 (eRF1) mouse monoclonal antibody, clone OTI2E3 (formerly 2E3)

Antibody type

Monoclonal

Description

ETF1 (eRF1) mouse monoclonal antibody, clone OTI2E3 (formerly 2E3)

Host

Mouse

Conjugate

Unconjugated

Epitope

ETF1

Isotype

IgG

Antibody clone number

OTI2E3

Vial size

100 µl

Concentration

0.97 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETF1 (RC220387 , Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ETF1.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ETF1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-ETF1 antibody(TA502891),(Red), compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Hela cells, using anti-ETF1 antibody(TA502891),(Red), compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC