NBP1-31113

antibody from Novus Biologicals

Targeting: ETF1 ERF, ERF1, RF1, SUP45L1, TB3-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-31113

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-31113, RRID:AB_2100535

Product name

Rabbit Polyclonal eRF1 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified.

Reactivity

Human, Mouse, Rat, Simian

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Chemical footprinting reveals conformational changes of 18S and 28S rRNAs at different steps of translation termination on the human ribosome.

Bulygin KN, Bartuli YS, Malygin AA, Graifer DM, Frolova LY, Karpova GG
RNA (New York, N.Y.) 2016 Feb;22(2):278-89

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Virus-mediated compartmentalization of the host translational machinery.

Desmet EA, Anguish LJ, Parker JS
mBio 2014 Sep 16;5(5):e01463-14

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: eRF1 Antibody [NBP1-31113] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with eRF1 antibody diluted at 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: eRF1 Antibody [NBP1-31113] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with eRF1 antibody diluted at 1:500.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: eRF1 Antibody [NBP1-31113] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: eRF1 Antibody [NBP1-31113] - Paraffin-embedded mouse prostate. eRF1 antibody diluted at 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: eRF1 Antibody [NBP1-31113] - Paraffin-embedded mouse testis. eRF1 antibody diluted at 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min