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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [4]
- Other assay [1]
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Validation data
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- Product number
- 18042-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CCNY Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MEFNPSDHP RASTIFLSKS QTDVREKRKS LFINHHPPGQ IARKYSSCST IFLDDSTVSQ PNLKYTIKCV ALAIYYHIKN RDPDGRMLLD IFDENLHPLS KSEVPPDYDK HNPEQKQIYR FVRTLFSAAQ LTAECAIVTL VYLERLLTYA EIDICPANWK RIVLGAILLA SKVWDDQAVW NVDYCQILKD ITVEDMNELE RQFLELLQFN INVPSSVYAK YYFDLRSLAE ANNLSFPLEP LSRERAHKLE AISRLCEDKY KDLRRSARKR SASADNLTLP RWSPAIIS (1-287 aa encoded by BC104773)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.21 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A549 cells were subjected to SDS PAGE followed by western blot with 18042-1-AP (CCNY antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 18042-1-AP (CCNY antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using CCNY antibody 18042-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using CCNY antibody 18042-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using CCNY antibody 18042-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed A549 cells using CCNY antibody (Product # 18042-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CCNY (IP:18042-1-AP, 3ug; Detection:18042-1-AP 1:600) with K-562 cells lysate 2400ug.
