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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- PAB14120 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB14120, RRID:AB_10677202
- Product name
- PHF8 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PHF8.
- Storage
- Store at -20°C. For long term storage store at -80°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- E14TG2A (mouse embryonic stem cells) were transfected with a conditional allele of PHF8.Nuclear extracts (135 ug) from wild type cells (WT) and from 2 clones (F1, B8) with an active and a targeted allele (F1ko, B8ko), respectively, were analysed by Western blot using PHF8 polyclonal antibody (Cat # PAB14120).The antibody was diluted 1 : 1,000.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA was performed using a serial dilution of PHF8 polyclonal antibody (Cat # PAB14120), crude serum and Flow Through in antigen coated wells.By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 6,500.
- Validation comment
- ELISA
