PA5-109289

antibody from Invitrogen Antibodies

Targeting: PHF8 JHDM1F, KDM7B, KIAA1111, ZNF422

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-109289

Provider

Invitrogen Antibodies

Product name

PHF8 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: PC-3M, A431, Hela, JAR, rat brain tissue , human lung cancer tissue, human colon tissue, mouse brain tissue, human kidney tissue, Siha.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PHF8 in Positive control: Lane 1: PC-3M, Lane 2: A431, Lane 3: Human kidney tissue. Samples were incubated with PHF8 polyclonal antibody (Product # PA5-109289), at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PHF8 in JAR cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PHF8 polyclonal antibody (Product # PA5-109289), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PHF8 in HeLa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PHF8 polyclonal antibody (Product # PA5-109289), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PHF8 in SiHa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PHF8 polyclonal antibody (Product # PA5-109289), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PHF8 in JAR cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PHF8 polyclonal antibody (Product # PA5-109289), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PHF8 in HeLa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PHF8 polyclonal antibody (Product # PA5-109289), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PHF8 in paraffin-embedded rat brain tissue. Samples were incubated with PHF8 polyclonal antibody (Product # PA5-109289), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PHF8 in paraffin-embedded mouse brain tissue. Samples were incubated with PHF8 polyclonal antibody (Product # PA5-109289), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PHF8 in paraffin-embedded human lung cancer tissue. Samples were incubated with PHF8 polyclonal antibody (Product # PA5-109289), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PHF8 in paraffin-embedded human colon tissue. Samples were incubated with PHF8 polyclonal antibody (Product # PA5-109289), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of PHF8 in SiHa cells (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with PHF8 polyclonal antibody (Product # PA5-109289) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.