A301-772A

antibody from Invitrogen Antibodies

Targeting: PHF8 JHDM1F, KDM7B, KIAA1111, ZNF422

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-772A

Provider

Invitrogen Antibodies

Product name

PHF8 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse PHF8 by western blot. Samples: Whole cell lysate (15 and 50 µg) from Jurkat, HeLa, HEK293T, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHF8 antibody (Product # A301-772A; lot A301-772A-4) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PHF8 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PHF8 antibody A301-772A (lot A301-772A-2) used for IP at 6 µg per reaction. PHF8 was also immunoprecipitated by a previous lot of this antibody (lot A301-772A-2) and rabbit anti-PHF8 antibody A303-773A For blotting immunoprecipitated PHF8, A301-772A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PHF8 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHF8 antibody A301-772A (lot A301-772A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse PHF8 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-PHF8 (Product # A301-772A; lot 4) used at a dilution of 1:200 (1 µg/mL). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PHF8 by immunohistochemistry. Samples: FFPE serial sections of human small cell lung cancer. Antibody: Affinity purified rabbit anti-PHF8 (Product # A301-772A lot 4) used at a dilution of 1:200 (1 µg/mL). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse PHF8 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- PHF8 (Cat. No. A301-772A Lot2) used at a dilution of 1:200 (1 µg/ml). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PHF8 by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PHF8 (Cat. No. A301-772A Lot2) used at a dilution of 1:200 (1 µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PHF8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PHF8 antibody A301-772A (lot A301-772A-4) used for IP at 6 µg per reaction. PHF8 was also immunoprecipitated by a previous lot of this antibody (lot A301-772A-3) and rabbit anti-PHF8 antibody A301-773A. For blotting immunoprecipitated PHF8, A301-772A was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 1 second.