A301-772A

antibody from Invitrogen Antibodies

Targeting: PHF8 JHDM1F, KDM7B, KIAA1111, ZNF422

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-772A

Provider

Invitrogen Antibodies

Product name

PHF8 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

The recommended shelf life for this product is 1 year from date of receipt.

Concentration

0.20 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse PHF8 by western blot. Samples: Whole cell lysate (15 and 50 µg) from Jurkat, HeLa, HEK293T, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHF8 antibody (Product # A301-772A; lot A301-772A-4) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PHF8 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PHF8 antibody A301-772A (lot A301-772A-2) used for IP at 6 µg per reaction. PHF8 was also immunoprecipitated by a previous lot of this antibody (lot A301-772A-2) and rabbit anti-PHF8 antibody A303-773A For blotting immunoprecipitated PHF8, A301-772A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PHF8 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHF8 antibody A301-772A (lot A301-772A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse PHF8 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-PHF8 (Product # A301-772A; lot 4) used at a dilution of 1:200 (1 µg/mL). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PHF8 by immunohistochemistry. Samples: FFPE serial sections of human small cell lung cancer. Antibody: Affinity purified rabbit anti-PHF8 (Product # A301-772A lot 4) used at a dilution of 1:200 (1 µg/mL). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse PHF8 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- PHF8 (Cat. No. A301-772A Lot2) used at a dilution of 1:200 (1 µg/ml). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PHF8 by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PHF8 (Cat. No. A301-772A Lot2) used at a dilution of 1:200 (1 µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PHF8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PHF8 antibody A301-772A (lot A301-772A-4) used for IP at 6 µg per reaction. PHF8 was also immunoprecipitated by a previous lot of this antibody (lot A301-772A-3) and rabbit anti-PHF8 antibody A301-773A. For blotting immunoprecipitated PHF8, A301-772A was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 1 second.