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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [4]
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- Product number
- NB100-93313 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-93313, RRID:AB_1237294
- Product name
- Rabbit Polyclonal PHF8 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Phf8 histone demethylase deficiency causes cognitive impairments through the mTOR pathway.
Chen X, Wang S, Zhou Y, Han Y, Li S, Xu Q, Xu L, Zhu Z, Deng Y, Yu L, Song L, Chen AP, Song J, Takahashi E, He G, He L, Li W, Chen CD
Nature communications 2018 Jan 9;9(1):114
Nature communications 2018 Jan 9;9(1):114
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PHF8 Antibody [NB100-93313] - Detection of Human PHF8 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHF8 antibody NB100-93313 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PHF8 Antibody [NB100-93313] - Detection of Human PHF8 on HeLa whole cell lysate using NB100-93313. PHF8 was also immunoprecipitated by rabbit anti-PHF8 antibody NB100-93314.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PHF8 Antibody [NB100-93313] - Detection of human and mouse PHF8 by western blot. Samples: Whole cell lysate (15 and 50 ug) from Jurkat, HeLa, HEK293T, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHF8 antibody NB100-93313 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: PHF8 Antibody [NB100-93313] - Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PHF8 used at a dilution of 1:200 (1 ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: PHF8 Antibody [NB100-93313] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- PHF8 used at a dilution of 1:200 (1 ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PHF8 Antibody [NB100-93313] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-PHF8 used at a dilution of 1:100.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PHF8 Antibody [NB100-93313] - FFPE section of mouse teratoma. Affinity purified rabbit anti-PHF8 used at a dilution of 1:100.
