Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- NBP1-33010 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-33010, RRID:AB_2084439
- Product name
- Rabbit Polyclonal CRYBA1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references The identification and characterization of the p.G91 deletion in CRYBA1 in a Chinese family with congenital cataracts.
Li D, Jing Q, Jiang Y
BMC medical genetics 2019 Sep 5;20(1):153
BMC medical genetics 2019 Sep 5;20(1):153
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CRYBA1 Antibody [NBP1-33010] - A. 50 ug mouse fetal eye lysate/extract 12 % SDS-PAGE beta A3 Crystallin antibody dilution: 1:10000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CRYBA1 Antibody [NBP1-33010] - The CRYBA1 p.G91del mutation reduced its expression in two cell lines. The protein levels of CRYBA1 were measured by Western blot. In 293T and SRA cells, the exogenous CRYBA1 was detected using anti-FLAG antibody; In SRA cells, the general CRYBA1 protein level was also measured using anti-CRYBA1 antibody. beta-Actin or GAPDH were used as internal control. NC, negative control: transfection reagent only. Image collected and cropped by CiteAb from the following publication (bmcmedgenet.biomedcentral.com/articles/10.1186/s12881-019-0882-z), licensed under a CC-BY licence.