PA5-57662

antibody from Invitrogen Antibodies

Targeting: SH3BP5 Sab

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-57662

Provider

Invitrogen Antibodies

Product name

SAB Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: ETQSVSSFSS GPTSPSEMPD QFPAVVRPGS LDLPSPVSLS EFGMMFPVLG PRSECSGASS PECEVERGDR AEGAENKTSD KANNNRG

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SAB in control (vector only transfected HEK293T lysate) and SH3BP5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a SAB Polyclonal Antibody (Product # PA5-57662).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SAB in human cell lines MCF-7 and Caco-2 using a SAB Polyclonal Antibody (Product # PA5-57662). Corresponding SH3BP5 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of SAB in human cell line A-431 using a SAB Polyclonal Antibody (Product # PA5-57662) shows localization to nucleoplasm and nuclear bodies.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of SAB in human testis tissue shows moderate cytoplasmic positivity in subsets of cells in seminiferus ducts. Samples were probed using a SAB Polyclonal Antibody (Product # PA5-57662).