Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A301-458A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDC2L5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CDC2L5 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, Jurkat, and KM12 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC2L5 antibody (Product # A301-458A-2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CDC2L5 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDC2L5 antibody A301-458A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. CDC2L5 was also immunoprecipitated by rabbit anti-CDC2L5 antibody BL5851, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 3 minutes (A) and 10 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CDC2L5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDC2L5 antibody (Product # A301-458A; Lot 2) used for IP at 6 µg per reaction. CDC2L5 was also immunoprecipitated by a previous lot of this antibody (Product # A301-458A; Lot 1) and a second antibody against a different epitope of CDC2L5 (BL5851). For blotting immunoprecipitated CDC2L5, Product # A301-458A was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
