H00006688-M01

antibody from Abnova Corporation

Targeting: SPI1 OF, PU.1, SFPI1, SPI-1, SPI-A

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00006688-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00006688-M01, RRID:AB_509202

Product name

SPI1 monoclonal antibody (M01), clone 1A3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant SPI1.

Antigen sequence

MEGFPLVPPPSEDLVPYDTDLYQRQTHEYYPYLSS
DGESHSDHYWDFHPHHVHSEFESFAENNFTELQSV
QPPQLQQLYRHMELEQMHVLDTPMVPPHPSLGHQV
SYLPRMCLQYPSLSP


Isotype

IgG

Antibody clone number

1A3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of SPI1 expression in transfected 293T cell line by SPI1 monoclonal antibody (M01), clone 1A3.Lane 1: SPI1 transfected lysate(31.083 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SPI1 is approximately 10ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol