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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00006688-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006688-M02, RRID:AB_922292
- Product name
- Mouse Monoclonal PU.1/Spi-1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. SPI1 - spleen focus forming virus (SFFV) proviral integration oncogene spi1 (2G1)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references The transcription factor IRF1 dictates the IL-21-dependent anticancer functions of TH9 cells.
Végran F, Berger H, Boidot R, Mignot G, Bruchard M, Dosset M, Chalmin F, Rébé C, Dérangère V, Ryffel B, Kato M, Prévost-Blondel A, Ghiringhelli F, Apetoh L
Nature immunology 2014 Aug;15(8):758-66
Nature immunology 2014 Aug;15(8):758-66
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PU.1/Spi-1 Antibody (2G1) [H00006688-M02] - SPI1 monoclonal antibody (M02), clone 2G1. Analysis of SPI1 expression in Raw 264.7.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: PU.1/Spi-1 Antibody (2G1) [H00006688-M02] - Detection limit for recombinant GST tagged SPI1 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: PU.1/Spi-1 Antibody (2G1) [H00006688-M02] - Analysis of monoclonal antibody to SPI1 on HeLa cell. Antibody concentration 10 ug/ml.
