Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-75738-20ug - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal PU.1/Spi-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 20 ug
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Analysis of THP-1 cell lysate using PU.1 Mouse Monoclonal Antibody (PU1/2146).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Formalin-fixed, paraffin-embedded human Lymph Node stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Formalin-fixed, paraffin-embedded human Spleen stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Flow Cytometric Analysis of PFA-fixed Ramos cells. PU.1/Spi-1 Antibody (PU1/2146) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
