IC5870P

antibody from R&D Systems

Targeting: SPI1 OF, PU.1, SFPI1, SPI-1, SPI-A

Antibody data

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Validation data

Product number: IC5870P - Provider product page
Provider: R&D Systems
Product name: Human PU.1/Spi-1 PE-conjugated Antibody
Antibody type: Polyclonal
Description: Antigen Affinity-purified. Detects human PU.1/Spi-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human SPI-B is observed.
Reactivity: Human
Host: Sheep
Antigen sequence: NP_001074016
Isotype: IgG
Vial size: 100 Tests
Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

SPI1 protein structure - IC5870P shown in red.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Detection of PU.1/Spi-1 in Th2-differentiated Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 5 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) and 10 μg/mL Goat Anti-Human IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA) for 5 days, then treated overnight with PMA and Calcium Ionomycin to induce Th2 differentiation were stained with Sheep Anti-Human PU.1/Spi-1 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5870P) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC016P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Detection of PU.1/Spi-1 in Human PBMC lymphocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with Sheep Anti-Human PU.1/Spi-1 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5870P) and Mouse Anti-Human CD3 epsilon PerCP-conjugated Monoclonal Antibody (Catalog # FAB100C). Quadrant markers were set based on control antibody staining (Catalog # IC016P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.