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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- TA502194 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502194, RRID:AB_11128796
- Product name
- DNAJB1 (Hsp40) mouse monoclonal antibody, clone OTI1E9 (formerly 1E9)
- Antibody type
- Monoclonal
- Description
- DNAJB1 (Hsp40) mouse monoclonal antibody, clone OTI1E9 (formerly 1E9)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- DNAJB1
- Isotype
- IgG
- Antibody clone number
- OTI1E9
- Vial size
- 100 µl
- Concentration
- 0.92 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNAJB1 (RC201762, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNAJB1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-DNAJB1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 1 cell line by using anti-DNAJB1 monoclonal antibody. (1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-DNAJB1 mouse monoclonal antibody (TA502194) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DNAJB1(RC201762).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DNAJB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502194)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC201762 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DNAJB1 antibody(TA502194), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-DNAJB1 antibody(TA502194),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
