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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA502195 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502195, RRID:AB_11125248
- Product name
- DNAJB1 (Hsp40) mouse monoclonal antibody, clone OTI1F9 (formerly 1F9)
- Antibody type
- Monoclonal
- Description
- DNAJB1 (Hsp40) mouse monoclonal antibody, clone OTI1F9 (formerly 1F9)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- DNAJB1
- Isotype
- IgG
- Antibody clone number
- OTI1F9
- Vial size
- 100 µl
- Concentration
- 1.6 mg/ml
Submitted references Tracking protein aggregation and mislocalization in cells with flow cytometry.
Ramdzan YM, Polling S, Chia CP, Ng IH, Ormsby AR, Croft NP, Purcell AW, Bogoyevitch MA, Ng DC, Gleeson PA, Hatters DM
Nature methods 2012 Mar 18;9(5):467-70
Nature methods 2012 Mar 18;9(5):467-70
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNAJB1 (RC201762, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNAJB1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DNAJB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-DNAJB1 mouse monoclonal antibody (TA502195) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DNAJB1(RC201762).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-DNAJB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502195)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC201762 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DNAJB1 antibody(TA502195), and then analyzed by flow cytometry.
- Validation comment
- FC
