13174-1-AP

antibody from Invitrogen Antibodies

Targeting: DNAJB1 Hsp40, HSPF1, RSPH16B, Sis1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

13174-1-AP

Provider

Invitrogen Antibodies

Product name

DNAJB1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MGKDYYQTL GLARGASDEE IKRAYRRQAL RYHPDKNKEP GAEEKFKEIA EAYDVLSDPR KREIFDRYGE EGLKGSGPSG GSGGGANGTS FSYTFHGDPH AMFAEFFGGR NPFDTFFGQR NGEEGMDIDD PFSGFPMGMG GFTNVNFGRS RSAQEPARKK QDPPVTHDLR VSLEEIYSGC TKKMKISHKR LNPDGKSIRN EDKILTIEVK KGWKEGTKIT FPKEGDQTSN NIPADIVFVL KDKPHNIFKR DGSDVIYPAR ISLREALCGC TVNVPTLDGR TIPVVFKDVI RPGMRRKVPG EGLPLPKTPE KRGDLIIEFE VIFPERIPQT SRTVLEQVLP I (1-340 aa encoded by BC019827)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.38 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 13174-1-AP (DNAJB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human liver tissue were subjected to SDS PAGE followed by western blot with 13174-1-AP (DNAJB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13174-1-AP (DNAJB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

COLO 320 cells were subjected to SDS PAGE followed by western blot with 13174-1-AP (DNAJB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 13174-1-AP (DNAJB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using DNAJB1 antibody 13174-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lymphoma using 13174-1-AP (DNAJB1 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lymphoma using 13174-1-AP (DNAJB1 antibody) at dilution of 1:100 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HeLa cells were intracellularly stained with 0.2 µg Anti-Human DNAJB1 (Product # 13174-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (green), and 0.2 µg Control Antibody. Cells were fixed with 90% MeOH.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-DNAJB1 (IP:13174-1-AP, 4ug; Detection:13174-1-AP 1:500) with mouse lung tissue lysate 4000ug.