Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- NBP1-03340 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-03340, RRID:AB_1522693
- Product name
- Rabbit Polyclonal WNK3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references With-No-Lysine Kinase 3 (WNK3) stimulates glioma invasion by regulating cell volume.
Haas BR, Cuddapah VA, Watkins S, Rohn KJ, Dy TE, Sontheimer H
American journal of physiology. Cell physiology 2011 Nov;301(5):C1150-60
American journal of physiology. Cell physiology 2011 Nov;301(5):C1150-60
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: WNK3 Antibody [NBP1-03340] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-WNK3 antibody used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).