AP6409B

antibody from Abgent

Targeting: FXN CyaY, FA, FARR, FRDA, X25

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP6409B

Provider

Abgent

Proper citation

Abgent Cat#AP6409b, RRID:AB_2105523

Product name

FXN Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of anti-FXN Antibody (C-term) (Cat#AP6409b) in 293 cell line lysates (35ug/lane). FXN (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of FXN (arrow) using rabbit polyclonal FXN Antibody (C-term) (RB12571). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FXN gene (Lane 2) (Origene Technologies).

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with FXN antibody (C-term) (Cat.#AP6409b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:10~50