45-6300

FXN antibody from Invitrogen Antibodies

CyaY, FA, FARR, FRDA, X25

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Recommended by provider

Immunohistochemistry

Data presented on provider website

Flow cytometry

Supportive data in Antibodypedia

Provider product page for 45-6300

Antibody Data

Product number

45-6300

Provider

Invitrogen Antibodies

Product name

Anti-FXN Monoclonal Antibody (18A5DB1)

Provider product page

Antibody type

Monoclonal

Antigen

Other

Description

Positive controls: Recombinant Human Frataxin; normal Human fibroblasts (MRC5); HL60 cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human colon adenocarcinoma.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

18A5DB1

Vial size

100 µg

Concentration

1 mg/mL

Storage

4° C, do not freeze

References

Long intronic GAA repeats causing Friedreich ataxia impede transcription elongation.
Punga T, Bühler M
EMBO molecular medicine 2010 Apr;2(4):120-9

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FXN in recombinant protein using a FXN Monoclonal antibody (Product # 45-6300) at a concentration of 5 µg/mL. 0.0005 µg of recombinant Human Frataxin was run in this analysis.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FXN in recombinant protein using a FXN Monoclonal antibody (Product # 45-6300) at a concentration of 5 µg/mL. 0.0005 µg of recombinant Human Frataxin was run in this analysis.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of FXN in HL60 cells using an FXN monoclonal antibody (Product # 45-6300) at 1 µg/mL is depicted by the blue line. The red line indicates an isotype control antibody.