MA3-085

FXN antibody from Invitrogen Antibodies

CyaY, FA, FARR, FRDA, X25

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Data presented on provider website

Flow cytometry

Supportive data in Antibodypedia

Provider product page for MA3-085

Antibody Data

Product number

MA3-085

Provider

Invitrogen Antibodies

Product name

Anti-FXN Monoclonal Antibody (NPM-1B2)

Provider product page

Antibody type

Monoclonal

Antigen

Other

Description

MA3-085 detects Frataxin from human and mouse samples. MA3-085 has been successfully used in Western blot, immunofluorescence, Flow Cytometry, and ELISA applications.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

NPM-1B2

Vial size

50 µL

Concentration

Conc. Not Determined

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Frataxin was performed by loading 20 µg of the indicated whole cell lysates and 5 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BX10). Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product # 62288), and blocked with 5% Milk in TBST for 1 hour at room temperature. Frataxin was detected at 23 kDa (human) and 19 kDa (mouse) using a Frataxin mouse monoclonal antibody (Product # MA3-085) at a dilution of 1:500 in blocking buffer for 1 hour at room temperature on a rocking platform, followed by a Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177) at a dilution of 1:1000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico substrate (Product # 34078) and the myECL Imager (Product # 62236).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of Frataxin was done on U2OS cells. Cells were fixed, permeabilized and stained with a Frataxin mouse monoclonal antibody (Product # MA3-081, blue histogram) at a dilution of 1:100. After incubation of the primary antibody on ice for an hour, the cells were stained with a Goat anti-mouse IgG Secondary Antibody, DyLight 680 conjugate (Product # 35519) at a dilution of 1:50 for 1 hour on ice. A representative 10,000 cells were acquired for each sample. The black histogram represents unstained control cells.