675903

antibody from BioLegend

Targeting: STING1 ERIS, FLJ38577, MITA, MPYS, NET23, STING, TMEM173

Immunocytochemistry (Data presented on provider website)

Immunoelectron microscopy (Supportive data in Antibodypedia)

Antibody Data

Product number

675903

Provider

BioLegend

Proper citation

BioLegend Cat#675903, RRID:AB_2566609

Product name

Alexa Fluor® 594 anti-STING (TMEM173)

Antibody type

Monoclonal

Antigen

Full length recombinant STING (TMEM173) protein produced in 293T cells.

Reactivity

Human

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

O94E12

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunoelectron microscopy

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

THP-1 cells were fixed with 4% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml Alexa Fluor® 594 conjugated (red) STING antibody (clone O94E12) in blocking buffer for two hours in room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.

Conjugate

Yellow dye