MAB7169
antibody from R&D Systems
Targeting: STING1
ERIS, FLJ38577, MITA, MPYS, NET23, STING, TMEM173
Antibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB7169 - Provider product page
- Provider
- R&D Systems
- Product name
- Human STING/TMEM173 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human STING/TMEM173 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q86WV6
- Isotype
- IgG
- Antibody clone number
- 723505
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The N-Terminal Domain of cGAS Determines Preferential Association with Centromeric DNA and Innate Immune Activation in the Nucleus.
PARP1 depletion induces RIG-I-dependent signaling in human cancer cells.
The deubiquitinase CYLD is a specific checkpoint of the STING antiviral signaling pathway.
HPV18 Persistence Impairs Basal and DNA Ligand-Mediated IFN-β and IFN-λ1 Production through Transcriptional Repression of Multiple Downstream Effectors of Pattern Recognition Receptor Signaling.
UBXN3B positively regulates STING-mediated antiviral immune responses.
Human cytomegalovirus-encoded US9 targets MAVS and STING signaling to evade type I interferon immune responses.
Extrachromosomal telomere repeat DNA is linked to ALT development via cGAS-STING DNA sensing pathway.
Evasion of the STING DNA-Sensing Pathway by VP11/12 of Herpes Simplex Virus 1.
Intracellular STING inactivation sensitizes breast cancer cells to genotoxic agents.
T cells detect intracellular DNA but fail to induce type I IFN responses: implications for restriction of HIV replication.
Pan-viral specificity of IFN-induced genes reveals new roles for cGAS in innate immunity.
HSV-1 degrades, stabilizes, requires, or is stung by STING depending on ICP0, the US3 protein kinase, and cell derivation.
Gentili M, Lahaye X, Nadalin F, Nader GPF, Puig Lombardi E, Herve S, De Silva NS, Rookhuizen DC, Zueva E, Goudot C, Maurin M, Bochnakian A, Amigorena S, Piel M, Fachinetti D, Londoño-Vallejo A, Manel N
Cell reports 2019 Feb 26;26(9):2377-2393.e13
Cell reports 2019 Feb 26;26(9):2377-2393.e13
PARP1 depletion induces RIG-I-dependent signaling in human cancer cells.
Ghosh R, Roy S, Franco S
PloS one 2018;13(3):e0194611
PloS one 2018;13(3):e0194611
The deubiquitinase CYLD is a specific checkpoint of the STING antiviral signaling pathway.
Zhang L, Wei N, Cui Y, Hong Z, Liu X, Wang Q, Li S, Liu H, Yu H, Cai Y, Wang Q, Zhu J, Meng W, Chen Z, Wang C
PLoS pathogens 2018 Nov;14(11):e1007435
PLoS pathogens 2018 Nov;14(11):e1007435
HPV18 Persistence Impairs Basal and DNA Ligand-Mediated IFN-β and IFN-λ1 Production through Transcriptional Repression of Multiple Downstream Effectors of Pattern Recognition Receptor Signaling.
Albertini S, Lo Cigno I, Calati F, De Andrea M, Borgogna C, Dell'Oste V, Landolfo S, Gariglio M
Journal of immunology (Baltimore, Md. : 1950) 2018 Mar 15;200(6):2076-2089
Journal of immunology (Baltimore, Md. : 1950) 2018 Mar 15;200(6):2076-2089
UBXN3B positively regulates STING-mediated antiviral immune responses.
Yang L, Wang L, Ketkar H, Ma J, Yang G, Cui S, Geng T, Mordue DG, Fujimoto T, Cheng G, You F, Lin R, Fikrig E, Wang P
Nature communications 2018 Jun 13;9(1):2329
Nature communications 2018 Jun 13;9(1):2329
Human cytomegalovirus-encoded US9 targets MAVS and STING signaling to evade type I interferon immune responses.
Choi HJ, Park A, Kang S, Lee E, Lee TA, Ra EA, Lee J, Lee S, Park B
Nature communications 2018 Jan 9;9(1):125
Nature communications 2018 Jan 9;9(1):125
Extrachromosomal telomere repeat DNA is linked to ALT development via cGAS-STING DNA sensing pathway.
Chen YA, Shen YL, Hsia HY, Tiang YP, Sung TL, Chen LY
Nature structural & molecular biology 2017 Dec;24(12):1124-1131
Nature structural & molecular biology 2017 Dec;24(12):1124-1131
Evasion of the STING DNA-Sensing Pathway by VP11/12 of Herpes Simplex Virus 1.
Deschamps T, Kalamvoki M
Journal of virology 2017 Aug 15;91(16)
Journal of virology 2017 Aug 15;91(16)
Intracellular STING inactivation sensitizes breast cancer cells to genotoxic agents.
Gaston J, Cheradame L, Yvonnet V, Deas O, Poupon MF, Judde JG, Cairo S, Goffin V
Oncotarget 2016 Nov 22;7(47):77205-77224
Oncotarget 2016 Nov 22;7(47):77205-77224
T cells detect intracellular DNA but fail to induce type I IFN responses: implications for restriction of HIV replication.
Berg RK, Rahbek SH, Kofod-Olsen E, Holm CK, Melchjorsen J, Jensen DG, Hansen AL, Jørgensen LB, Ostergaard L, Tolstrup M, Larsen CS, Paludan SR, Jakobsen MR, Mogensen TH
PloS one 2014;9(1):e84513
PloS one 2014;9(1):e84513
Pan-viral specificity of IFN-induced genes reveals new roles for cGAS in innate immunity.
Schoggins JW, MacDuff DA, Imanaka N, Gainey MD, Shrestha B, Eitson JL, Mar KB, Richardson RB, Ratushny AV, Litvak V, Dabelic R, Manicassamy B, Aitchison JD, Aderem A, Elliott RM, García-Sastre A, Racaniello V, Snijder EJ, Yokoyama WM, Diamond MS, Virgin HW, Rice CM
Nature 2014 Jan 30;505(7485):691-5
Nature 2014 Jan 30;505(7485):691-5
HSV-1 degrades, stabilizes, requires, or is stung by STING depending on ICP0, the US3 protein kinase, and cell derivation.
Kalamvoki M, Roizman B
Proceedings of the National Academy of Sciences of the United States of America 2014 Feb 4;111(5):E611-7
Proceedings of the National Academy of Sciences of the United States of America 2014 Feb 4;111(5):E611-7
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human STING/TMEM173 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # MAB7169) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for STING/TMEM173 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- STING/TMEM173 in U937 Human Cell Line. STING/TMEM173 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Mouse Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # MAB7169) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of STING/TMEM173 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # MAB7169, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.