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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00079075-B01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00079075-B01P, RRID:AB_1673931
- Product name
- DSCC1 purified MaxPab mouse polyclonal antibody (B01P)
- Antibody type
- Polyclonal
- Description
- Mouse polyclonal antibody raised against a full-length human DSCC1 protein.
- Antigen sequence
MKRTRDEVDATLQIAKLNAAELLPAVHCLGFGPGA
SGAAAGDFCLLELEPTLCQQLEDGHSLVIRGDKDE
QAVLCSKDKTYDLKIADTSNMLLFIPGCKTPDQLK
KEDSHCNIIHTEIFGFSNNYWELRRRRPKLKKLKK
LLMENPYEGPDSQKEKDSNSSKYTTEDLLDQIQAS
EEEIMTQLQVLNACKIGGYWRILEFDYEMKLLNHV
TQLVDSESWSFGKVPLNTCLQELGPLEPEEMIEHC
LKCYGKKYVDEGEVYFELDADKICRAAARMLLQNA
VKFNLAEFQEVWQQSVPEGMVTSLDQLKGLALVDR
HSRPEIIFLLKVDDLPEDNQERFNSLFSLREKWTE
EDIAPYIQDLCGEKQTIGALLTKYSRSSMQNGVKV
YNSRRPIS- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Overexpression of cohesion establishment factor DSCC1 through E2F in colorectal cancer.
Yamaguchi K, Yamaguchi R, Takahashi N, Ikenoue T, Fujii T, Shinozaki M, Tsurita G, Hata K, Niida A, Imoto S, Miyano S, Nakamura Y, Furukawa Y
PloS one 2014;9(1):e85750
PloS one 2014;9(1):e85750
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of DSCC1 expression in transfected 293T cell line (H00079075-T01) by DSCC1 MaxPab polyclonal antibody.Lane 1: DCC1 transfected lysate(43.23 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- DSCC1 MaxPab polyclonal antibody. Western Blot analysis of DSCC1 expression in A-431.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to DCC1 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
