Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [12]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 15519-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HACL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: AHAEESIKK LVEQYKLPFL PTPMGKGVVP DNHPYCVGAA RSRALQFADV IVLFGARLNW ILHFGLPPRY QPDVKFIQVD ICAEELGNNV KPAVTLLGNI HAVTKQLLEE LDKTPWQYPP ESKWWKTLRE KMKSNEAASK ELASKKSLPM NYYTVFYHVQ EQLPRDCFVV SEGANTMDIG RTVLQNYLPR HRLDAGTFGT MGVGLGFAIA AAVVAKDRSP GQWIICVEGD SAFGFSGMEV ETICRYNLPI ILLVVNNNGI YQGFDTDTWK EMLKFQDATA VVPPMCLLPN SHYEQVMTAF GGKGYFVQTP EELQKSLRQS LADTTKPSLI NIM (227-558 aa encoded by BC001627)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.33 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 15519-1-AP (HACL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of HACL1 antibody (15519-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HACL1 transfected HEK-293 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 15519-1-AP (HACL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 15519-1-AP (HACL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded rat liver using 15519-1-AP (HACL1 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded rat liver slide using 15519-1-AP (HACL1 Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded rat liver using 15519-1-AP (HACL1 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded rat kidney using 15519-1-AP (HACL1 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded rat kidney using 15519-1-AP (HACL1 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 15519-1-AP (HACL1 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 15519-1-AP (HACL1 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skeletal muscle using 15519-1-AP (HACL1 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skeletal muscle using 15519-1-AP (HACL1 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded rat liver slide using 15519-1-AP (HACL1 Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 15519-1-AP (HACL1 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 15519-1-AP (HACL1 antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-HACL1 (IP:15519-1-AP, 4ug; Detection:15519-1-AP 1:300) with HeLa cells lysate 2640 ug.
