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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-28511 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HACL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, mouse liver. Predicted reactivity: Mouse (87%), Rat (86%), Pig (86%), Chimpanzee (100%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HACL1 Polyclonal Antibody (Product # PA5-28511). Sample (30 µg whole cell lysate). A: 293T. 7.5% SDS PAGE. HACL1 Polyclonal Antibody (Product # PA5-28511) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HACL1 Polyclonal Antibody (Product # PA5-28511). Sample (50 µg of whole cell lysate). Lane A: mouse liver . 7.5% SDS PAGE. HACL1 Polyclonal Antibody (Product # PA5-28511) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HACL1 in paraformaldehyde-fixed HeLa cells using a HACL1 polyclonal antibody (Product # PA5-28511) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HACL1 Polyclonal Antibody detects HACL1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. HACL1 Polyclonal Antibody (Product # PA5-28511) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HACL1 Polyclonal Antibody detects HACL1 protein at cytosol on human normal liver by immunohistochemical analysis. Sample: Paraffin-embedded human normal liver. HACL1 Polyclonal Antibody (Product # PA5-28511) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
