H00005813-M01

antibody from Abnova Corporation

Targeting: PURA PUR-ALPHA, PUR1, PURALPHA

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005813-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005813-M01, RRID:AB_1112080

Product name

PURA monoclonal antibody (M01), clone 3F10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant PURA.

Antigen sequence

TQGQTIALPAQGLIEFRDALAKLIDDYGVEEEPAE
LPEGTSLTVDNKRFFFDVGSNKYGVFMRVSEVKPT
YRNSITVPYKVWAKFGHTFCKYSEETKKIQEKQRE
KRAAC


Isotype

IgG

Antibody clone number

3F10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

PURA monoclonal antibody (M01), clone 3F10. Western Blot analysis of PURA expression in human skeletal muscle.

Protocol

Protocol

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

PURA monoclonal antibody (M01), clone 3F10. Western Blot analysis of PURA expression in rat brain.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PURA is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to PURA on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol