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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- PA5-26069 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BPI Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The Mucosal Antibacterial Response Profile and Fecal Microbiota Composition Are Linked to the Disease Course in Patients with Newly Diagnosed Ulcerative Colitis.
Magnusson MK, Strid H, Isaksson S, Simrén M, Öhman L
Inflammatory bowel diseases 2017 Jun;23(6):956-966
Inflammatory bowel diseases 2017 Jun;23(6):956-966
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a BPI polyclonal antibody (Product # PA5-26069) in HL-60 cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue using a BPI polyclonal antibody (Product # PA5-26069), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HL-60 cells using a BPI polyclonal antibody (Product # PA5-26069) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 4. Localization of BPI in the epithelial cell layer and crypts. Biopsy specimens from rectum stained with polyclonal rabbit-anti-BPI-IgG followed by goat-anti-rabbit-AlexaFluor488. Sections were mounted with 4', 6-Diamidino-2-Phenylindole Dihydrochloride (DAPI) (chromosome stain) and visualized at x20 magnification, green = BPI stain, blue = nuclear stain (DAPI). A representative staining from a patient with newly diagnosed UC is shown.
