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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- MAB6756 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB6756, RRID:AB_10676587
- Product name
- HBEGF monoclonal antibody, clone 4G10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against recombinant HBEGF.
- Isotype
- IgG
- Antibody clone number
- 4G10
- Storage
- Store at -20°C. For long term storage store at -80°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Heparin-binding EGF-like growth factor is a promising target for ovarian cancer therapy.
Heparin-binding EGF-like growth factor, which acts as the diphtheria toxin receptor, forms a complex with membrane protein DRAP27/CD9, which up-regulates functional receptors and diphtheria toxin sensitivity.
Miyamoto S, Hirata M, Yamazaki A, Kageyama T, Hasuwa H, Mizushima H, Tanaka Y, Yagi H, Sonoda K, Kai M, Kanoh H, Nakano H, Mekada E
Cancer research 2004 Aug 15;64(16):5720-7
Cancer research 2004 Aug 15;64(16):5720-7
Heparin-binding EGF-like growth factor, which acts as the diphtheria toxin receptor, forms a complex with membrane protein DRAP27/CD9, which up-regulates functional receptors and diphtheria toxin sensitivity.
Iwamoto R, Higashiyama S, Mitamura T, Taniguchi N, Klagsbrun M, Mekada E
The EMBO journal 1994 May 15;13(10):2322-30
The EMBO journal 1994 May 15;13(10):2322-30
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blotting with HBEGF monoclonal antibody, clone 4G10 (Cat # MAB6756).Lane 1. Vero cell extract.2. Vero cells carrrying human HBEGF expression vector.3. Vero cells carrying mouse HB-EGF expression vector.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunocytochemical staining with HBEGF monoclonal antibody, clone 4G10 (Cat # MAB6756).A. VERO cells carrying human HGEGF expression vector.B. Vero cells carrying mouse HB-EGF expression vector.Cells treated with antibody 4G10, fixed with 4% PFA and reacted with Cys3 conjugated 2nd antibody.
- Validation comment
- Immunocytochemistry
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation with HBEGF monoclonal antibody, clone 4G10 (Cat # MAB6756).Lane 1. Vero cell extract.2. Vero cells carrrying human HBEGF expression vector.3. Vero cells carrying mouse HB-EGF expression vector.Except that the cell surface was biotinylated.After immunoprecipitation with the antibody, HRP-conjugated streptoavidin was used to detect the HBEGF by Western blotting.
- Validation comment
- Immunoprecipitation
