500-P329-1MG

antibody from Invitrogen Antibodies

Targeting: HBEGF DTR, DTS, HEGFL

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

500-P329-1MG

Provider

Invitrogen Antibodies

Product name

HBEGF Polyclonal Antibody, PeproTech®

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

AA Sequence of recombinant protein: DLQEADLDLL RVTLSSKPQA LATPNKEEHG KRKKKGKGLG KKRDPCLRKY KDFCIHGECK YVKELRAPSC ICHPGYHGER CHGLSL.

Concentration

0.1-1.0 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect Human HB-EGF by Western Blot analysis HBEGF Polyclonal Antibody (Product # 500-P329-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human HB-EGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect Human HB-EGF by Western Blot analysis HBEGF Polyclonal Antibody (Product # 500-P329-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human HB-EGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA: To detect Human HB-EGF by sandwich ELISA (using 100 µL/well), a concentration of 0.25-1.0 µg/mL of HBEGF Polyclonal Antibody (Product # 500-P329-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech HBEGF Polyclonal Antibody, Biotin (Product # 500-P329BT-1MG) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human HB-EGF.