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- Antibody Data
- Antigen structure
- References [25]
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- Validations
- Immunohistochemistry [2]
- Blocking/Neutralizing [1]
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- Product number
- AF-259-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human HB-EGF Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human HB-EGF in ELISAs and Western blots. In direct ELISAs, less than 1% cross reactivity with recombinant mouse HB-EGF is observed. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) Amphiregulin, rhBetacellulin, rhEpiregulin, and recombinant mouse Epigen is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q99075- Isotype
- IgG
- Vial size
- 250 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Osmotic Induction of Angiogenic Growth Factor Expression in Human Retinal Pigment Epithelial Cells.
M2 macrophages induce ovarian cancer cell proliferation via a heparin binding epidermal growth factor/matrix metalloproteinase 9 intercellular feedback loop.
Specific bone cells produce DLL4 to generate thymus-seeding progenitors from bone marrow.
MicroRNA-132 enhances transition from inflammation to proliferation during wound healing.
A mouse model of otitis media identifies HB-EGF as a mediator of inflammation-induced mucosal proliferation.
Heparin-binding epidermal growth factor-like growth factor eliminates constraints on activated Kras to promote rapid onset of pancreatic neoplasia.
An EGFR wild type-EGFRvIII-HB-EGF feed-forward loop regulates the activation of EGFRvIII.
Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions.
Notch increases the shedding of HB-EGF by ADAM12 to potentiate invadopodia formation in hypoxia.
Immature doublecortin-positive hippocampal neurons are important for learning but not for remembering.
Heparin binding epidermal growth factor in renal ischaemia/reperfusion injury.
Amphiregulin carboxy-terminal domain is required for autocrine keratinocyte growth.
Wounding-induced synthesis of hyaluronic acid in organotypic epidermal cultures requires the release of heparin-binding egf and activation of the EGFR.
HER family receptor abnormalities in lung cancer brain metastases and corresponding primary tumors.
EGFR signaling is required for TGF-beta 1 mediated COX-2 induction in human bronchial epithelial cells.
Expression and role of EGFR ligands induced in airway cells by PM2.5 and its components.
Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration.
Ras activation in response to phorbol ester proceeds independently of the EGFR via an unconventional nucleotide-exchange factor system in COS-7 cells.
Expression of heparin-binding epidermal growth factor-like growth factor and its receptors in the human fallopian tube and endometrium after treatment with mifepristone.
Human trophoblast survival at low oxygen concentrations requires metalloproteinase-mediated shedding of heparin-binding EGF-like growth factor.
Role of epidermal growth factor receptor transactivation in alpha1B-adrenoceptor phosphorylation.
Trafficking of nuclear heparin-binding epidermal growth factor-like growth factor into an epidermal growth factor receptor-dependent autocrine loop in response to oxidative stress.
Autocrine extracellular signal-regulated kinase (ERK) activation in normal human keratinocytes: metalloproteinase-mediated release of amphiregulin triggers signaling from ErbB1 to ERK.
Heparin-binding EGF-like growth factor regulates human extravillous cytotrophoblast development during conversion to the invasive phenotype.
Heparin-binding EGF-like growth factor regulates human extravillous cytotrophoblast development during conversion to the invasive phenotype.
Veltmann M, Hollborn M, Reichenbach A, Wiedemann P, Kohen L, Bringmann A
PloS one 2016;11(1):e0147312
PloS one 2016;11(1):e0147312
M2 macrophages induce ovarian cancer cell proliferation via a heparin binding epidermal growth factor/matrix metalloproteinase 9 intercellular feedback loop.
Carroll MJ, Kapur A, Felder M, Patankar MS, Kreeger PK
Oncotarget 2016 Dec 27;7(52):86608-86620
Oncotarget 2016 Dec 27;7(52):86608-86620
Specific bone cells produce DLL4 to generate thymus-seeding progenitors from bone marrow.
Yu VW, Saez B, Cook C, Lotinun S, Pardo-Saganta A, Wang YH, Lymperi S, Ferraro F, Raaijmakers MH, Wu JY, Zhou L, Rajagopal J, Kronenberg HM, Baron R, Scadden DT
The Journal of experimental medicine 2015 May 4;212(5):759-74
The Journal of experimental medicine 2015 May 4;212(5):759-74
MicroRNA-132 enhances transition from inflammation to proliferation during wound healing.
Li D, Wang A, Liu X, Meisgen F, Grünler J, Botusan IR, Narayanan S, Erikci E, Li X, Blomqvist L, Du L, Pivarcsi A, Sonkoly E, Chowdhury K, Catrina SB, Ståhle M, Landén NX
The Journal of clinical investigation 2015 Aug 3;125(8):3008-26
The Journal of clinical investigation 2015 Aug 3;125(8):3008-26
A mouse model of otitis media identifies HB-EGF as a mediator of inflammation-induced mucosal proliferation.
Suzukawa K, Tomlin J, Pak K, Chavez E, Kurabi A, Baird A, Wasserman SI, Ryan AF
PloS one 2014;9(7):e102739
PloS one 2014;9(7):e102739
Heparin-binding epidermal growth factor-like growth factor eliminates constraints on activated Kras to promote rapid onset of pancreatic neoplasia.
Ray KC, Moss ME, Franklin JL, Weaver CJ, Higginbotham J, Song Y, Revetta FL, Blaine SA, Bridges LR, Guess KE, Coffey RJ, Crawford HC, Washington MK, Means AL
Oncogene 2014 Feb 13;33(7):823-31
Oncogene 2014 Feb 13;33(7):823-31
An EGFR wild type-EGFRvIII-HB-EGF feed-forward loop regulates the activation of EGFRvIII.
Li L, Chakraborty S, Yang CR, Hatanpaa KJ, Cipher DJ, Puliyappadamba VT, Rehman A, Jiwani AJ, Mickey B, Madden C, Raisanen J, Burma S, Saha D, Wang Z, Pingle SC, Kesari S, Boothman DA, Habib AA
Oncogene 2014 Aug 14;33(33):4253-64
Oncogene 2014 Aug 14;33(33):4253-64
Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions.
Smith AO, Bowers SL, Stratman AN, Davis GE
PloS one 2013;8(12):e85147
PloS one 2013;8(12):e85147
Notch increases the shedding of HB-EGF by ADAM12 to potentiate invadopodia formation in hypoxia.
Díaz B, Yuen A, Iizuka S, Higashiyama S, Courtneidge SA
The Journal of cell biology 2013 Apr 15;201(2):279-92
The Journal of cell biology 2013 Apr 15;201(2):279-92
Immature doublecortin-positive hippocampal neurons are important for learning but not for remembering.
Vukovic J, Borlikova GG, Ruitenberg MJ, Robinson GJ, Sullivan RK, Walker TL, Bartlett PF
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 Apr 10;33(15):6603-13
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 Apr 10;33(15):6603-13
Heparin binding epidermal growth factor in renal ischaemia/reperfusion injury.
Mulder GM, Nijboer WN, Seelen MA, Sandovici M, Bos EM, Melenhorst WB, Trzpis M, Kloosterhuis NJ, Visser L, Henning RH, Leuvenink HG, Ploeg RJ, Sunnarborg SW, van Goor H
The Journal of pathology 2010 Jun;221(2):183-92
The Journal of pathology 2010 Jun;221(2):183-92
Amphiregulin carboxy-terminal domain is required for autocrine keratinocyte growth.
Stoll SW, Johnson JL, Li Y, Rittié L, Elder JT
The Journal of investigative dermatology 2010 Aug;130(8):2031-40
The Journal of investigative dermatology 2010 Aug;130(8):2031-40
Wounding-induced synthesis of hyaluronic acid in organotypic epidermal cultures requires the release of heparin-binding egf and activation of the EGFR.
Monslow J, Sato N, Mack JA, Maytin EV
The Journal of investigative dermatology 2009 Aug;129(8):2046-58
The Journal of investigative dermatology 2009 Aug;129(8):2046-58
HER family receptor abnormalities in lung cancer brain metastases and corresponding primary tumors.
Sun M, Behrens C, Feng L, Ozburn N, Tang X, Yin G, Komaki R, Varella-Garcia M, Hong WK, Aldape KD, Wistuba II
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Aug 1;15(15):4829-37
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Aug 1;15(15):4829-37
EGFR signaling is required for TGF-beta 1 mediated COX-2 induction in human bronchial epithelial cells.
Liu M, Yang SC, Sharma S, Luo J, Cui X, Peebles KA, Huang M, Sato M, Ramirez RD, Shay JW, Minna JD, Dubinett SM
American journal of respiratory cell and molecular biology 2007 Nov;37(5):578-88
American journal of respiratory cell and molecular biology 2007 Nov;37(5):578-88
Expression and role of EGFR ligands induced in airway cells by PM2.5 and its components.
Rumelhard M, Ramgolam K, Hamel R, Marano F, Baeza-Squiban A
The European respiratory journal 2007 Dec;30(6):1064-73
The European respiratory journal 2007 Dec;30(6):1064-73
Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration.
Li Y, Fan J, Chen M, Li W, Woodley DT
The Journal of investigative dermatology 2006 Sep;126(9):2096-105
The Journal of investigative dermatology 2006 Sep;126(9):2096-105
Ras activation in response to phorbol ester proceeds independently of the EGFR via an unconventional nucleotide-exchange factor system in COS-7 cells.
Rubio I, Rennert K, Wittig U, Beer K, Dürst M, Stang SL, Stone J, Wetzker R
The Biochemical journal 2006 Sep 1;398(2):243-56
The Biochemical journal 2006 Sep 1;398(2):243-56
Expression of heparin-binding epidermal growth factor-like growth factor and its receptors in the human fallopian tube and endometrium after treatment with mifepristone.
Sun XX, Gemzell-Danielsson K, Li HZ, Stâbi B, Stavreus-Evers A
Fertility and sterility 2006 Jan;85(1):171-8
Fertility and sterility 2006 Jan;85(1):171-8
Human trophoblast survival at low oxygen concentrations requires metalloproteinase-mediated shedding of heparin-binding EGF-like growth factor.
Armant DR, Kilburn BA, Petkova A, Edwin SS, Duniec-Dmuchowski ZM, Edwards HJ, Romero R, Leach RE
Development (Cambridge, England) 2006 Feb;133(4):751-9
Development (Cambridge, England) 2006 Feb;133(4):751-9
Role of epidermal growth factor receptor transactivation in alpha1B-adrenoceptor phosphorylation.
Casas-González P, García-Sáinz JA
European journal of pharmacology 2006 Aug 7;542(1-3):31-6
European journal of pharmacology 2006 Aug 7;542(1-3):31-6
Trafficking of nuclear heparin-binding epidermal growth factor-like growth factor into an epidermal growth factor receptor-dependent autocrine loop in response to oxidative stress.
Kim J, Adam RM, Freeman MR
Cancer research 2005 Sep 15;65(18):8242-9
Cancer research 2005 Sep 15;65(18):8242-9
Autocrine extracellular signal-regulated kinase (ERK) activation in normal human keratinocytes: metalloproteinase-mediated release of amphiregulin triggers signaling from ErbB1 to ERK.
Kansra S, Stoll SW, Johnson JL, Elder JT
Molecular biology of the cell 2004 Sep;15(9):4299-309
Molecular biology of the cell 2004 Sep;15(9):4299-309
Heparin-binding EGF-like growth factor regulates human extravillous cytotrophoblast development during conversion to the invasive phenotype.
Leach RE, Kilburn B, Wang J, Liu Z, Romero R, Armant DR
Developmental biology 2004 Feb 15;266(2):223-37
Developmental biology 2004 Feb 15;266(2):223-37
Heparin-binding EGF-like growth factor regulates human extravillous cytotrophoblast development during conversion to the invasive phenotype.
Leach RE, Kilburn B, Wang J, Liu Z, Romero R, Armant DR
Developmental biology 2004 Feb 15;266(2):223-37
Developmental biology 2004 Feb 15;266(2):223-37
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- HB-EGF in Human Placenta. HB-EGF was detected in immersion fixed paraffin-embedded sections of human placenta using 15 µg/mL Human HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-259-NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- HB-EGF in Human Placenta. HB-EGF was detected in immersion fixed paraffin-embedded sections of human placenta using Human HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-259-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by HB-EGF and Neutralization by Human HB-EGF Antibody. Recombinant Human HB-EGF (Catalog # 259-HE) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human HB-EGF (20 ng/mL) is neutralized (green line) by increasing concentrations of Human HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-259-NA). The ND50 is typically 0.1-0.6 µg/mL.
